Hughes celebrated with the crowd but was soon crestfallen as he was delivered the news. The head-on footage of the race looked conclusive that there had been interference.
It is understood the England team unsuccessfully counter-protested the decision.
Hughes’ disqualification meant Northern Ireland’s Leon Reid, who had finished fourth in the race, was bumped up to a bronze medal while Canada’s Aaron Brown moved up to the silver medal.
IAAF president Lord Sebastian Coe, commenting on the Seven Network, believed Hughes should have been disqualified but predicted a counter-appeal by the English team.
“You can run on the line and you could be 3m ahead and run on the line, there is no material advantage, but if you do happen to impede or clash that will be the argument,” Coe said.